exl protein specifically binds BLE1, a bicoid mRNA localization element, and is required for one phase of its activity.
نویسندگان
چکیده
منابع مشابه
RNA regulatory element BLE1 directs the early steps of bicoid mRNA localization.
Deployment of the bicoid morphogen gradient in early Drosophila embryos requires the prelocalization of bicoid mRNA to the anterior pole of the egg. This anterior localization is mediated by a cis-acting localization signal contained within the 3' untranslated region of the bicoid mRNA. Here we use a series of bicoid transgenes carrying small deletions in the 3' untranslated region to survey fo...
متن کاملCom, the phage Mu mom translational activator, is a zinc-binding protein that binds specifically to its cognate mRNA.
Bacteriophage Mu controls an unusual DNA-modification function encoded by the mom gene, which is located in an operon that consists of two overlapping genes. The com gene, located proximal to the 5' end of the common mRNA transcript, encodes a polypeptide of 62 amino acids that is required for translation of mom. Analysis of the derived amino acid sequence reveals that Com contains zinc-binding...
متن کاملA new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.
In yeast Saccharomyces cerevisiae, Ash1p, a protein determinant for mating-type switching, is segregated within the daughter cell nucleus to establish asymmetry of HO expression. The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where translation occurs. To study the mechanism regulating ASH1 mRNA tran...
متن کاملDistinguishing direct from indirect roles for bicoid mRNA localization factors.
Localization of bicoid mRNA to the anterior of the Drosophila oocyte is essential for patterning the anteroposterior body axis in the early embryo. bicoid mRNA localizes in a complex multistep process involving transacting factors, molecular motors and cytoskeletal components that remodel extensively during the lifetime of the mRNA. Genetic requirements for several localization factors, includi...
متن کاملThe Drosophila synaptotagmin-like protein bitesize is required for growth and has mRNA localization sequences within its open reading frame.
The vertebrate synaptotagmin-like protein granuphilin binds to the vesicle-trafficking proteins Rab27a and Munc18 and can modulate exocytosis of insulin-containing secretory granules in pancreatic beta cell lines. Here, we report the molecular and genetic characterization of bitesize, a granuphilin homolog and the only Drosophila synaptotagmin-like protein. Mutations that affect bitesize have r...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.23.10787